Establishment and maintenance of cell lines
The development of monoclonal antibody cell lines ensures that they are stable in continuous culture, can be routinely cultured to high cell concentrations and produce consistent amounts of active specific antibody.
- All cell lines are tested for mycoplasma infection.
- Stability of cell lines is monitored by limiting dilution cloning analysis.
- The best clone is isolated according to growth rate, antibody potency and antibody concentration.
- A master cell bank and a working cell bank are frozen indefinitely in liquid nitrogen.
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